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http://hdl.handle.net/11547/11566
Title: | In Vitro Efficacy of Curcumin-Loaded Amine-Functionalized Mesoporous Silica Nanoparticles against MCF-7 Breast Cancer Cells |
Authors: | Mohebian, Zahra |
Keywords: | HTERT GENE-EXPRESSION EFFECTIVE GROWTH-INHIBITION |
Issue Date: | 2023 |
Series/Report no.: | 13;2 |
Abstract: | Purpose: Mesoporous silica nanoparticles (MSNs) have drawn substantial interest as drug nanocarriers for breast cancer therapy. Nevertheless, because of the hydrophilic surfaces, the loading of well-known hydrophobic polyphenol anticancer agent curcumin (Curc) into MSNs is usually very low.Methods: For this purpose, Curc molecules were loaded into amine-functionalized MSNs (MSNs-NH2-Curc) and characterized using thermal gravimetric analysis (TGA), Fourier -transform infrared (FTIR), field emission scanning electron microscope (FE-SEM), transmission electron microscope (TEM), Brunauer-Emmett-Teller (BET). MTT assay and confocal microscopy, respectively, were used to determine the cytotoxicity and cellular uptake of the MSNs-NH2- Curc in the MCF-7 breast cancer cells. Besides, the expression levels of apoptotic genes were evaluated via quantitative polymerase chain reaction (qPCR) and western blot.Results: It was revealed that MSNs-NH2 possessed high values of drug loading efficiency and exhibited slow and sustained drug release compared to bare MSNs. According to the MTT findings, while the MSNs-NH2-Curc were nontoxic to the human non-tumorigenic MCF-10A cells at low concentrations, it could considerably decrease the viability of MCF-7 breast cancer cells compared to the free Curc in all concentrations after 24, 48 and 72 hours exposure times. A cellular uptake study using confocal fluorescence microscopy confirmed the higher cytotoxicity of MSNs-NH2-Curc in MCF-7 cells. Further, it was found that the MSNs-NH2-Curc could drastically affect the mRNA and protein levels of Bax, Bcl-2, caspase 3, caspase 9, and hTERT relative to the free Curc treatment.Conclusion: Taken together, these preliminary results suggest the amine-functionalized MSNs-based drug delivery platform as a promising alternative approach for Curc loading and safe breast cancer treatment. |
URI: | http://hdl.handle.net/11547/11566 |
ISSN: | 2228-5881 |
Appears in Collections: | Web Of Science |
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apb-13-317.pdf | 1.91 MB | Adobe PDF | View/Open |
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